Determination of Helicobacter pylori virulence-associated genes in duodenal ulcer and gastric biopsies

Authors

  • Abazar Pournajaf Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
  • Behzad Emadi Department of Microbiology, School of Medicine, Iran University of Medical Sciences, International campus, Tehran, Iran.
  • Mahmoud Khodabandeh Department of Pediatric Infectious Disease, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
  • Mehrdad Gholami Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
  • Ramazan Rajabnia Infectious Diseases and Tropical Medicine Research Center, Babol University of Medical Sciences, Babol, Iran.
  • Sajjad Yaghoubi Division of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
  • Yasaman Saeidi Department of Microbiology, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran.
Abstract:

Background: Helicobacter pylori (H. pylori or Hp) has been strongly associated with the peptic ulcer diseases, chronic gastritis, ulcers, and gastric cancer. Genes associated with pathogenicity have been designated for H. pylori, and some of them appear to be related to more severe clinical consequences of the infection. The present study was conducted to determine cagA, vacA, cagE, iceA1, oipA, and iceA2 genes in H. pylori strains isolated from gastroduodenal patients, who referred to Shariati hospital in Tehran, Iran. &nbsp;&nbsp; Methods: Gastric biopsy specimens were collected during endoscopy from patients, who referred to the Shariati hospital in Tehran, Iran during January and November 2015. After isolation of H. pylori from the biopsy culture, genomic DNA was extracted and subsequently used to identify H. pylori and virulence genes using specific primers. &nbsp;&nbsp; Results: The isolation rate of H. pylori strains was 65.7% (169/257). The frequency of cagA, vacA, cagE, iceA1, oipA, and iceA2 was 143 (% 84.6), 169 (100%), 131 (77.5%), 97 (57.3%), 89 (52.6%), and 72 (42.6%), respectively. &nbsp;&nbsp; Conclusion: In this study, a significant difference was observed between investigated genes and strains isolated from PUD and GC patients (p<0.05). &nbsp; &nbsp;

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Journal title

volume 31  issue 1

pages  555- 559

publication date 2017-01

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